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The specificities of three monoclonal antibodies (MAbs) that bind to murine preimplantation mouse embryos in the zygote, morula, and blastocyst stages were examined. These MAbs were produced against embryos microinjected with dextran-conjugated antibodies. Out of nine MAbs raised against morulae, six were able to bind to mouse zygotes and these showed different patterns of labeling. Five MAbs raised against blastocysts exhibited cross-reactivity with embryos in morulae but only one stained zygotes and these were able to induce complement-dependent damage to embryos. The staining patterns of zygotes stained with a MAb against the zona pellucida resembled those of MAb against the zona pellucida. The staining patterns of morulae stained with these MAbs differed from those of staining patterns of these same MAbs against other murine embryos and cross-reacting staining patterns on these MAbs with mouse embryos microinjected with anti-mouse IgG. It was concluded that these three MAbs recognize different epitopes on mouse preimplantation embryos and three of these MAbs that bind to embryos in the zygote and early blastocyst stages cannot be used to identify the developmental stage of these early embryos.PORT ADELAIDE will play three home games at the club’s new Netball South Australia centre at Salisbury as part of the venue’s three-year trial.

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